ARG62906

anti-CD62L / L-Selectin antibody [LT-TD180] (FITC)

anti-CD62L / L-Selectin antibody [LT-TD180] (FITC) for Flow cytometry and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [LT-TD180] recognizes CD62L / L-Selectin
Tested Reactivity Hu
Tested Application FACS
Specificity The clone LT-TD180 reacts with CD62L (L-selectin), a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
Host Mouse
Clonality Monoclonal
Clone LT-TD180
Isotype IgG1
Target Name CD62L / L-Selectin
Immunogen Peripheral blood leukocytes
Conjugation FITC
Alternate Names Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6402 Human SELL

Swiss-port # P14151 Human L-selectin

Gene Symbol SELL
Gene Full Name selectin L
Background CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Function Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 42 kDa

Images (2) Click the Picture to Zoom In

  • ARG62906 anti-CD62L / L-Selectin antibody [LT-TD180] (FITC) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG62906 anti-CD62L / L-Selectin antibody [LT-TD180] (FITC).

  • ARG62906 anti-CD62L / L-Selectin antibody [LT-TD180] (FITC) FACS image

    Flow Cytometry: Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62906 anti-CD62L / L-Selectin antibody [LT-TD180] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).

Clone References

Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO).

Linnebacher M et al.
J Biomed Biotechnol.,  (2010)

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NKp80 defines and stimulates a reactive subset of CD8 T cells.

Kuttruff S et al.
Blood.,  (2009)

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The frequency of regulatory CD3+CD8+CD28- CD25+ T lymphocytes in human peripheral blood increases with age.

Simone R et al.
J Leukoc Biol.,  (2008)

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