ARG62905
anti-CD62L / L-Selectin antibody [DREG56]
anti-CD62L / L-Selectin antibody [DREG56] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [DREG56] recognizes CD62L / L-Selectin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, FuncSt, IHC-Fr, IP, WB |
| Specificity | The clone DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. HLDA V; WS Code S056 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DREG56 |
| Isotype | IgG1 |
| Target Name | CD62L / L-Selectin |
| Antigen Species | Human |
| Immunogen | PMA-activated human peripheral blood leukocytes |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. Functional studies: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | SELL |
| Gene Full Name | selectin L |
| Background | CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells. |
| Function | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt] |
| Highlight | Related products: CD62L antibodies; CD62L ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 42 kDa |
Images (2) Click the Picture to Zoom In
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ARG62905 anti-CD62L / L-Selectin antibody [DREG56] FACS image
Flow Cytometry: Human peripheral blood stained with ARG62905 anti-CD62L / L-Selectin antibody [DREG56] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62905 anti-CD62L / L-Selectin antibody [DREG56] FACS image
Flow Cytometry: Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62905 anti-CD62L / L-Selectin antibody [DREG56] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin.
Critical role of Lck in L-selectin signaling induced by sulfatides engagement.
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep.
