ARG62426
anti-CD6 antibody [SPV-L14]
anti-CD6 antibody [SPV-L14] for ELISA,Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [SPV-L14] recognizes CD6 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, FACS, ICC/IF, IHC, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SPV-L14 |
| Isotype | IgG1 |
| Target Name | CD6 |
| Antigen Species | Human |
| Immunogen | Anti-CD6 antibody was raised against CD8+ cytotoxic T-cell clone |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD6; TP120; T-cell differentiation antigen CD6; T12 |
Application Instructions
| Application Note | IHC: 0.5-1.0 ug/ml FACS: 0.5-1.0 ug antibody/million cells ICC/IF: 0.5-1.0 ug/ml IP: 0.5-1.0 ug antibody/500 ug protein lysate * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A/G purified |
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30203 Human T-cell differentiation antigen CD6 Swiss-port # Q61003 Mouse T-cell differentiation antigen CD6 |
|---|---|
| Gene Symbol | CD6 |
| Gene Full Name | CD6 molecule |
| Background | CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular “scavenger receptor cysteine-rich” (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease. |
| Function | Involved in cell adhesion. Binds to CD166. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 72 kDa |
| PTM | After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues. Glycosylated. |
