ARG53874
anti-CD54 / ICAM1 antibody [MEM-111] (PE)
anti-CD54 / ICAM1 antibody [MEM-111] (PE) for Flow cytometry and Human,Rat,Bovine
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [MEM-111] recognizes CD54 / ICAM1 |
|---|---|
| Tested Reactivity | Hu, Rat, Bov |
| Tested Application | FACS |
| Specificity | The clone MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-111 |
| Isotype | IgG2a |
| Target Name | CD54 / ICAM1 |
| Antigen Species | Human |
| Immunogen | Raji human Burkitt's lymphoma cell line |
| Conjugation | PE |
| Alternate Names | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ICAM1 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Background | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 58 kDa |
| PTM | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
Images (1) Click the Picture to Zoom In
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ARG53874 anti-CD54 / ICAM1 antibody [MEM-111] (PE) FACS image
Flow Cytometry: Activated Human peripheral blood stained with ARG53874 anti-CD54 / ICAM1 antibody [MEM-111] (PE).
Clone References
Suramin: a potential therapy for diabetic nephropathy.
Rhinovirus upregulates transient receptor potential channels in a human neuronal cell line: implications for respiratory virus-induced cough reflex sensitivity.
Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin.
ELISA / Human
The use of scaffold-free cell sheet technique to refine mesenchymal stromal cell-based therapy for heart failure.
Neutrophil serine proteases mediate inflammatory cell recruitment by glomerular endothelium and progression towards dysfunction.
Attenuation of Notch signaling promotes the differentiation of neural progenitors into neurons in the hippocampal CA1 region after ischemic injury.
Multipotent Adult Progenitor Cells (MAPC) contribute to hepatocarcinoma neovasculature.
FACS / Horse
