ARG62887
anti-CD54 / ICAM1 antibody [MEM-111]
anti-CD54 / ICAM1 antibody [MEM-111] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Bovine
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-111] recognizes CD54 / ICAM1 |
|---|---|
| Tested Reactivity | Hu, Rat, Bov |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | The clone MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-111 |
| Isotype | IgG2a |
| Target Name | CD54 / ICAM1 |
| Antigen Species | Human |
| Immunogen | Raji human Burkitt's lymphoma cell line |
| Conjugation | Un-conjugated |
| Alternate Names | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: Raji, K562, JY and activated T-lymphocytes. IHC-P: Thymus and RE cells |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ICAM1 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Background | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30035 Phospho CD54 / ICAM1 Antibody Duo (Total, pY512) Related products: CD54 antibodies; CD54 ELISA Kits; CD54 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 58 kDa |
| PTM | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
Images (1) Click the Picture to Zoom In
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ARG62887 anti-CD54 / ICAM1 antibody [MEM-111] WB image
Western blot: 1. TNF-alpha activated HUVEC cell lysate, 2. HUVEC cell lysate stained with ARG62887 anti-CD54 / ICAM1 antibody [MEM-111].
Lower bands represent tubulin as a loading control.
Clone References
Suramin: a potential therapy for diabetic nephropathy.
Rhinovirus upregulates transient receptor potential channels in a human neuronal cell line: implications for respiratory virus-induced cough reflex sensitivity.
Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin.
ELISA / Human
The use of scaffold-free cell sheet technique to refine mesenchymal stromal cell-based therapy for heart failure.
Multipotent Adult Progenitor Cells (MAPC) contribute to hepatocarcinoma neovasculature.
FACS / Horse
