ARG62885
anti-CD54 / ICAM1 antibody [1H4] (azide free)
anti-CD54 / ICAM1 antibody [1H4] (azide free) for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [1H4] recognizes CD54 / ICAM1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | The clone 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1H4 |
| Isotype | IgG2b |
| Target Name | CD54 / ICAM1 |
| Antigen Species | Human |
| Immunogen | Raji cells and spleen cells fused with NS1 cells |
| Conjugation | Un-conjugated |
| Alternate Names | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ICAM1 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Background | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 58 kDa |
| PTM | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
Clone References
Dendritic cell-lymphocyte cross talk downregulates host restriction factor SAMHD1 and stimulates HIV-1 replication in dendritic cells.
Phagocytosis of dying tumor cells by human peritoneal mesothelial cells.
Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes.
FACS / Human
Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation.
ICC/IF / Human
