ARG22608
anti-CD54 / ICAM1 antibody [1A29] (FITC)
anti-CD54 / ICAM1 antibody [1A29] (FITC) for Flow cytometry and Rat
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [1A29] recognizes CD54 / ICAM1 This antibody recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily.CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (Etienne, S. et al.).Functionally 1A29 inhibits homotypic aggregation of PHA blasts. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A29 |
| Isotype | IgG1 |
| Target Name | CD54 / ICAM1 |
| Antigen Species | Rat |
| Immunogen | Rat Ax cells (a HEV derived cell line). |
| Conjugation | FITC |
| Alternate Names | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Icam1 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Background | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 58 kDa |
| PTM | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
Images (3) Click the Picture to Zoom In
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ARG22608 anti-CD54 / ICAM1 antibody [1A29] (FITC) FACS image
Flow Cytometry: Stimulated Rat spleen cells stained with ARG22608 anti-CD54 / ICAM1 antibody [1A29] (FITC).
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ARG22608 anti-CD54 / ICAM1 antibody [1A29] (FITC) FACS image
Flow Cytometry: Figure A. ARG22608 anti-CD54 / ICAM1 antibody [1A29] (FITC) and RPE conjugated Mouse IgG1 isotype control. Figure B. ARG22608 anti-CD54 / ICAM1 antibody [1A29] (FITC) and RPE conjugated Mouse anti Rat CD161. All experiments performed on red cell lysed Rat blood gated on mononuclear cells.
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ARG22608 anti-CD54 / ICAM1 antibody [1A29] (FITC) FACS image
Flow Cytometry: Figure A. RPE conjugated Mouse anti Rat CD161 and FITC conjugated Mouse IgG1 isotype control. Figure B. RPE conjugated Mouse anti Rat CD161 and ARG22608 anti-CD54 / ICAM1 antibody [1A29] (FITC). All experiments performed on red cell lysed Rat blood gated on mononuclear cells.
