ARG23345

anti-CD53 antibody [TS53] (azide free)

anti-CD53 antibody [TS53] (azide free) for Flow cytometry,Immunoprecipitation,Western blot and Human

Overview

Product Description Azide free Mouse Monoclonal antibody [TS53] recognizes CD53
Tested Reactivity Hu
Tested Application FACS, IP, WB
Specificity This antibody recognizes the MEM-53 antigen, a 30-50 kDa (smear) protein.
Host Mouse
Clonality Monoclonal
Clone TS53
Isotype IgG1
Target Name CD53
Antigen Species Human
Immunogen Jurkat cell line
Conjugation Un-conjugated
Alternate Names Tetraspanin-25; Leukocyte surface antigen CD53; Tspan-25; Cell surface glycoprotein CD53; CD antigen CD53; MOX44; TSPAN25

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing conditions.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 30-40 kDa

Properties

Form Liquid
Purification Note Sterile-filtered through 0.22 µm.
Buffer PBS
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 963 Human CD53

Swiss-port # P19397 Human Leukocyte surface antigen CD53

Gene Symbol CD53
Gene Full Name CD53 molecule
Background The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Function Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity). [UniProt]
Calculated MW 24 kDa

Images (1) Click the Picture to Zoom In

  • ARG23345 anti-CD53 antibody [TS53] (azide free) WB image

    Western blot: Raji cells were lysed (lysis buffer containing 1% Brij97 or triton) and stained with ARG23345 anti-CD53 antibody [TS53] (azide free) under non-reducing conditions.