ARG62881
anti-CD52 antibody [HI186]
anti-CD52 antibody [HI186] for CyTOF®-candidate,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [HI186] recognizes CD52 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, IHC-P |
| Specificity | The clone HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract. HLDA VI; WS Code BP 523 HLDA VI; WS Code T 6T-057 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HI186 |
| Isotype | IgG2b |
| Target Name | CD52 |
| Antigen Species | Human |
| Immunogen | Human tonsil |
| Conjugation | Un-conjugated |
| Alternate Names | He5; CAMPATH-1 antigen; Human epididymis-specific protein 5; Cambridge pathology 1 antigen; CDw52; Epididymal secretory protein E5; CDW52; CD antigen CD52 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD52 |
| Gene Full Name | CD52 molecule |
| Background | CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis. |
| Function | May play a role in carrying and orienting carbohydrate, as well as having a more specific role. [UniProt] |
| Highlight | Related products: CD52 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Immune System antibody |
| Calculated MW | 7 kDa |
Images (2) Click the Picture to Zoom In
-
ARG62881 anti-CD52 antibody [HI186] FACS image
Flow Cytometry: Patient sample with Acute Lymphoblastic Leukemia (CD52 positive cALL) stained with ARG62881 anti-CD52 antibody [HI186], followed by incubation with PE-labelled secondary antibody.
-
ARG62881 anti-CD52 antibody [HI186] FACS image
Flow Cytometry: Patient sample with Acute Lymphoblastic Leukemia (CD52 positive cALL) stained with ARG62881 anti-CD52 antibody [HI186], followed by incubation with PE-labelled secondary antibody.
