ARG56036
anti-CD50 / ICAM3 antibody [101-1D2]
anti-CD50 / ICAM3 antibody [101-1D2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
Product Description | Mouse Monoclonal antibody [101-1D2] recognizes CD50 / ICAM3 |
---|---|
Tested Reactivity | Hu |
Tested Application | FACS, ICC/IF, IHC-P |
Host | Mouse |
Clonality | Monoclonal |
Clone | 101-1D2 |
Isotype | IgG2a, kappa |
Target Name | CD50 / ICAM3 |
Antigen Species | Human |
Immunogen | Stimulated Human leukocytes. |
Conjugation | Un-conjugated |
Alternate Names | CD50; CD antigen CD50; Intercellular adhesion molecule 3; ICAM-3; ICAM-R; CDw50; CDW50 |
Application Instructions
Application Suggestion |
|
||||||||
---|---|---|---|---|---|---|---|---|---|
Application Note | Antigen retrieval for IHC-P: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
---|---|
Purification | Purification with Protein G. |
Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
Preservative | 0.05% Sodium azide |
Stabilizer | 0.1 mg/ml BSA |
Concentration | 0.2 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
---|---|
Gene Symbol | ICAM3 |
Gene Full Name | intercellular adhesion molecule 3 |
Background | The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008] |
Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. [UniProt] |
Cellular Localization | Cell surface |
Calculated MW | 60 kDa |
PTM | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |