ARG56036

anti-CD50 / ICAM3 antibody [101-1D2]

anti-CD50 / ICAM3 antibody [101-1D2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Mouse Monoclonal antibody [101-1D2] recognizes CD50 / ICAM3
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone 101-1D2
Isotype IgG2a, kappa
Target Name CD50 / ICAM3
Antigen Species Human
Immunogen Stimulated Human leukocytes.
Conjugation Un-conjugated
Alternate Names CD50; CD antigen CD50; Intercellular adhesion molecule 3; ICAM-3; ICAM-R; CDw50; CDW50

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells in 0.1ml
ICC/IF0.5 - 1 µg/ml
IHC-P0.5 - 1 µg/ml
Application Note Antigen retrieval for IHC-P: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3385 Human ICAM3

Swiss-port # P32942 Human Intercellular adhesion molecule 3

Gene Symbol ICAM3
Gene Full Name intercellular adhesion molecule 3
Background The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. [UniProt]
Cellular Localization Cell surface
Calculated MW 60 kDa
PTM Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.