ARG23397
anti-CD5 antibody [YKIX322.3] (PE)
anti-CD5 antibody [YKIX322.3] (PE) for Flow cytometry and Dog
Overview
| Product Description | PE-conjugated Rat Monoclonal antibody [YKIX322.3] recognizes CD5 Rat anti Dog CD5 antibody, clone YKIX322.3 recognizes canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huang et al. 2008). Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Villiers 2002). |
|---|---|
| Tested Reactivity | Dog |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YKIX322.3 |
| Isotype | IgG2a |
| Target Name | CD5 |
| Antigen Species | Dog |
| Immunogen | Concanavilin A activated canine peripheral blood cells |
| Conjugation | PE |
| Alternate Names | CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD5 |
|---|---|
| Gene Full Name | CD5 molecule |
| Function | May act as a receptor in regulating T-cell proliferation. [UniProt] |
| Calculated MW | 55 kDa |
| PTM | Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG23397 anti-CD5 antibody [YKIX322.3] (PE) FACS image
Flow Cytometry: Figure A. A647 conjugated Rat anti Canine CD4 and PE-conjugated Rat IgG2a isotype control. Figure B. A647 conjugated Rat anti Canine CD4 and ARG23397 anti-CD5 antibody [YKIX322.3] (PE). All experiments performed on red cell lysed Canine blood gated on mononuclear cells.
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ARG23397 anti-CD5 antibody [YKIX322.3] (PE) FACS image
Flow Cytometry: Figure A. ARG23397 anti-CD5 antibody [YKIX322.3] (PE) and A647 conjugated Rat IgG2a isotype control. Figure B. ARG23397 anti-CD5 antibody [YKIX322.3] (PE) and A647 conjugated Rat anti Canine CD4. All experiments performed on red cell lysed Canine blood gated on mononuclear cells.
