ARG23396

anti-CD5 antibody [YKIX322.3] (FITC)

anti-CD5 antibody [YKIX322.3] (FITC) for Flow cytometry and Dog

Overview

Product Description FITC-conjugated Rat Monoclonal antibody [YKIX322.3] recognizes CD5
Rat anti Dog CD5 antibody, clone YKIX322.3 recognizes canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huang et al. 2008). Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Villiers 2002).
Tested Reactivity Dog
Tested Application FACS
Host Rat
Clonality Monoclonal
Clone YKIX322.3
Isotype IgG2a
Target Name CD5
Antigen Species Dog
Immunogen Concanavilin A activated canine peripheral blood cells
Conjugation FITC
Alternate Names CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl of whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD5
Gene Full Name CD5 molecule
Function May act as a receptor in regulating T-cell proliferation. [UniProt]
Calculated MW 55 kDa
PTM Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. [UniProt]