ARG22173
anti-CD49f / Integrin alpha 6 antibody [4F10]
anti-CD49f / Integrin alpha 6 antibody [4F10] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody [4F10] recognizes CD49f / Integrin alpha 6 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-Fr, IHC-P, IP |
| Specificity | Human CD49f |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4F10 |
| Isotype | IgG2b, kappa |
| Target Name | CD49f / Integrin alpha 6 |
| Antigen Species | Human |
| Immunogen | SW1222 Human colorectal cell line |
| Conjugation | Un-conjugated |
| Alternate Names | CD49 antigen-like family member F; ITGA6B; Alpha6p; VLA-6; CD antigen CD49f; CD49f; Integrin alpha-6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA6 |
| Gene Full Name | integrin, alpha 6 |
| Background | The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. [UniProt] |
| Calculated MW | 127 kDa |
| PTM | Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand. In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro. Palmitoylation by DHHC3 enhances stability and cell surface expression. |
Clone References
Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells
FACS / Human
Remodeling of the human endometrial epithelium is regulated by laminin and type IV collagen
FACS / Human
Laminin-332 and α3β1 integrin-supported migration of bronchial epithelial cells is modulated by fibronectin
ICC/IF / Human
Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting tetraspan interactions
IP / Human
Association between integrin expression and prognosis in localized prostate cancer
IHC-P / Human
Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis
2D / Human
Mechanical stimulation induces rapid fibroblast proliferation and accelerates the early maturation of human skin substitutes
ICC/IF / Human
