ARG23224
anti-CD49f / Integrin alpha 6 antibody [450-30A]
anti-CD49f / Integrin alpha 6 antibody [450-30A] for Flow cytometry,IHC-Frozen sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [450-30A] recognizes CD49f / Integrin alpha 6 Mouse anti Human CD49f antibody, clone 450-30A recognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f. CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 450-30A |
| Isotype | IgG1 |
| Target Name | CD49f / Integrin alpha 6 |
| Antigen Species | Human |
| Immunogen | Alpha 6 beta 4 integrin purified from A431 cells. |
| Conjugation | Un-conjugated |
| Alternate Names | CD49 antigen-like family member F; ITGA6B; Alpha6p; VLA-6; CD antigen CD49f; CD49f; Integrin alpha-6 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA6 |
| Gene Full Name | integrin, alpha 6 |
| Background | The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. [UniProt] |
| Calculated MW | 127 kDa |
| PTM | Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand. In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro. Palmitoylation by DHHC3 enhances stability and cell surface expression. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG23224 anti-CD49f / Integrin alpha 6 antibody [450-30A] FACS image
Flow Cytometry: Human peripheral blood lymphocytes stained with ARG23224 anti-CD49f / Integrin alpha 6 antibody [450-30A].
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ARG23224 anti-CD49f / Integrin alpha 6 antibody [450-30A] WB image
Western blot: Jurkat whole cell lysate stained with ARG23224 anti-CD49f / Integrin alpha 6 antibody [450-30A].
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ARG23224 anti-CD49f / Integrin alpha 6 antibody [450-30A] FACS image
Flow Cytometry: Human peripheral blood monocytes stained with ARG23224 anti-CD49f / Integrin alpha 6 antibody [450-30A].
