ARG23695
anti-CD49b / Integrin alpha 2 antibody [AK7]
anti-CD49b / Integrin alpha 2 antibody [AK7] for Flow cytometry,Functional study,IHC-Frozen sections and Human,Baboon,Rhesus Monkey
Overview
| Product Description | Mouse Monoclonal antibody [AK7] recognizes CD49b / Integrin alpha 2. Clone AK7 recognizes the integrin alpha 2 subunit, a ~160 kDa glycoprotein that non-covalently associates with the ~130 kDa integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines. Mouse anti Human CD49b antibody, clone AK7 inhibits cell attachment to collagen. |
|---|---|
| Tested Reactivity | Hu, Bb, R. Mk |
| Tested Application | FACS, FuncSt, IHC-Fr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AK7 |
| Isotype | IgG1 |
| Target Name | CD49b / Integrin alpha 2 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | Collagen receptor; VLA-2 subunit alpha; HPA-5; CD49B; CD49 antigen-like family member B; GPIa; VLA-2; CD antigen CD49b; BR; VLAA2; Platelet membrane glycoprotein Ia; Integrin alpha-2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ITGA2 |
| Gene Full Name | integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) |
| Background | This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Function | Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. [UniProt] |
| Calculated MW | 129 kDa |
