ARG62419
anti-CD48 antibody [B333 (5-4.8)]
anti-CD48 antibody [B333 (5-4.8)] for Flow cytometry,ICC/IF,IHC-Frozen sections,Western blot and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [B333 (5-4.8)] recognizes CD48 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, WB |
| Specificity | Reacts with CD48, a 45 kD glycosylphosphatidylinositol linked cell surface molecules. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B333 (5-4.8) |
| Isotype | IgG2a |
| Target Name | CD48 |
| Antigen Species | Human |
| Immunogen | Human PBL cell line |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen; BCM1 surface antigen; CD antigen CD48; TCT.1; BLAST1; BCM1; MEM-102; mCD48; Signaling lymphocytic activation molecule 2; BLAST; hCD48; SLAMF2; SLAM family member 2 |
Application Instructions
| Application Note | FACS: 1-5µg for 106 cells. ICC/IF: 5 - 20 µg/ml. IHC-Fr: 5 - 20 µg/ml. WB: 1 - 5 µg/ml. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | Human Tonsil and Lymph Node |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | PBS (pH 8.0) and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD48 |
| Gene Full Name | CD48 molecule |
| Background | This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Function | Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 28 kDa |
