ARG62871
anti-CD47 antibody [MEM-122]
anti-CD47 antibody [MEM-122] for Flow cytometry,IHC-Frozen sections,Western blot and Human,Primates,Pig
Cancer antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-122] recognizes CD47 |
|---|---|
| Tested Reactivity | Hu, NHuPrm, Pig |
| Tested Application | FACS, IHC-Fr, WB |
| Specificity | The clone MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. HLDA VI; WS Code AS A051 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-122 |
| Isotype | IgM |
| Target Name | CD47 |
| Immunogen | COS-7 (African green monkey) cells |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by CHT-chromatography and precipitation methods. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD47 |
| Gene Full Name | CD47 molecule |
| Background | CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina. |
| Function | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt] |
| Research Area | Cancer antibody; Immune System antibody |
| Calculated MW | 35 kDa |
Images (1) Click the Picture to Zoom In
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ARG62871 anti-CD47 antibody [MEM-122] FACS image
Flow Cytometry: Porcine peripheral blood stained with ARG62871 anti-CD47 antibody [MEM-122], followed by incubation with FITC-labelled secondary antibody.
Panel A was stained with Isotype mouse IgM control.
