ARG65549

anti-CD45R0 antibody [UCHL1]

anti-CD45R0 antibody [UCHL1] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody

Overview

Product Description Mouse Monoclonal antibody [UCHL1] recognizes CD45R0
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, IHC-Fr, IHC-P, IP
Specificity The clone UCHL1 recognizes CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.
HLDA III; WS Code NL 826
HLDA III; WS Code T 128
HLDA IV; WS Code NL 31
HLDA V; WS Code BP BP460
HLDA V; WS Code T T-081
HLDA V; WS Code T T-CD45.43
Host Mouse
Clonality Monoclonal
Clone UCHL1
Isotype IgG2a
Target Name CD45R0
Antigen Species Human
Immunogen Human IL-2 dependent T cells
Conjugation Un-conjugated
Alternate Names LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS10 µg/ml
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 μl of the suggested working dilution to label 10^6 cells in 100 μl.
IHC-P: Pretreatment: This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P and IHC-Fr: Tonsil

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-G affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5788 Human PTPRC

Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C

Gene Symbol PTPRC
Gene Full Name protein tyrosine phosphatase, receptor type, C
Background CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Function CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
Highlight Related products:
CD45R0 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Calculated MW 147 kDa
PTM Heavily N- and O-glycosylated.

Clone References

Immune monitoring of the circulation and the tumor microenvironment in patients with regionally advanced melanoma receiving neoadjuvant ipilimumab.

IHC-P / Human

Tarhini AA et al.
PLoS One.,  (2014)

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Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.

IHC / Human

Zlobec I et al.
J Transl Med.,  (2013)

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Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers.

ICC/IF / Human

Carolan BJ et al.
Cancer Res.,  (2006)

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Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

Smith SH et al.
Immunology.,  (1986)

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Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.

IHC-Fr, IHC-P / 

Norton AJ et al.
J Clin Pathol.,  (1986)

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