ARG62860
anti-CD45R / B220 antibody [IVA103]
anti-CD45R / B220 antibody [IVA103] for Flow cytometry,ICC/IF,Immunoprecipitation and Bovine
Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [IVA103] recognizes CD45R |
|---|---|
| Tested Reactivity | Bov |
| Tested Application | FACS, ICC/IF, IP |
| Specificity | The clone IVA103 reacts with CD45R antigen (bovine), a single chain type I transmembrane glycoprotein (member of the protein tyrosine phosphatase family), a restricted form of the CD45 family. CD45R is an essential regulator of T and B lymphocytes antigen receptor signaling and it is specifically expressed in hematopoietic cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | IVA103 |
| Isotype | IgG1 |
| Target Name | CD45R / B220 |
| Immunogen | Bovine leukocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PTPRC |
| Gene Full Name | protein tyrosine phosphatase, receptor type, C |
| Background | CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
| Function | CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt] |
| Highlight | Related products: CD45R antibodies; CD45R Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| Research Area | Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody |
| Calculated MW | 147 kDa |
| PTM | Heavily N- and O-glycosylated. |
Images (3) Click the Picture to Zoom In
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ARG62860 anti-CD45R / B220 antibody [IVA103] ICC/IF image
Immunofluorescence: Acetone / methanol-fixed Bovine peripheral blood (lymphocyte-enriched suspension) stained with ARG62860 anti-CD45R / B220 antibody [IVA103] (green), DAPI (blue) for nuclear staining.
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ARG62860 anti-CD45R / B220 antibody [IVA103] FACS image
Flow Cytometry: Separation of bovine CD45R positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Bovine peripheral whole blood stained with ARG62860 anti-CD45R / B220 antibody [IVA103] at 0.1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62860 anti-CD45R / B220 antibody [IVA103] FACS image
Flow Cytometry: Bovine peripheral whole blood stained with ARG62860 anti-CD45R / B220 antibody [IVA103] at 0.1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
