ARG23038

anti-CD45 antibody [OX-1] (PE)

anti-CD45 antibody [OX-1] (PE) for Flow cytometry and Rat

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [OX-1] recognizes CD45
Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.This product is routinely tested in flow cytometry on rat splenocytes
Tested Reactivity Rat
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone OX-1
Isotype IgG1
Target Name CD45
Antigen Species Rat
Immunogen Rat thymocyte membrane glycoproteins.
Conjugation PE
Alternate Names LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Unpurified.
Buffer PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose
Preservative 0.09% Sodium azide
Stabilizer 1% BSA and 5% Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24699 Rat PTPRC

Swiss-port # P04157 Rat Receptor-type tyrosine-protein phosphatase C

Gene Symbol Ptprc
Gene Full Name protein tyrosine phosphatase, receptor type, C
Background CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Function CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
Calculated MW 147 kDa
PTM Heavily N- and O-glycosylated.

Images (2) Click the Picture to Zoom In

  • ARG23038 anti-CD45 antibody [OX-1] (PE) FACS image

    Flow Cytometry: Rat spleen cells stained with ARG23038 anti-CD45 antibody [OX-1] (PE).

  • ARG23038 anti-CD45 antibody [OX-1] (PE) FACS image

    Flow Cytometry: Figure A. ARG23038 anti-CD45 antibody [OX-1] (PE) and FITC conjugated mouse IgG2a isotype control. Figure B. ARG23038 anti-CD45 antibody [OX-1] (PE) and FITC conjugated mouse anti rat CD26. All experiments performed on red cell lysed rat blood gated on mononuclear cells.