ARG62850

anti-CD44 antibody [MEM-85] (Biotin)

anti-CD44 antibody [MEM-85] (Biotin) for Flow cytometry and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [MEM-85] recognizes CD44
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
HLDA IV; WS Code NL 706
HLDA VI; WS Code AS Ref.15
Host Mouse
Clonality Monoclonal
Clone MEM-85
Isotype IgG2b
Target Name CD44
Immunogen Leukocytes of a patient suffering from LGL Type Leukaemia.
Conjugation Biotin
Alternate Names MDU2; MDU3; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Extracellular matrix receptor III; PGP-I; Epican; CDW44; Phagocytic glycoprotein 1; Pgp1; HUTCH-I; MC56; Hyaluronate receptor; CD antigen CD44; Heparan sulfate proteoglycan; CD44 antigen; LHR; IN; HCELL; Phagocytic glycoprotein I; PGP-1; CSPG8; MIC4; ECMR-III; CDw44

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 960 Human CD44

Swiss-port # P16070 Human CD44 antigen

Gene Symbol CD44
Gene Full Name CD44 molecule (Indian blood group)
Background CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody
Calculated MW 82 kDa
PTM Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

Images (1) Click the Picture to Zoom In

  • ARG62850 anti-CD44 antibody [MEM-85] (Biotin) FACS image

    Flow Cytometry: Human peripheral blood leukocytes stained with ARG62850 anti-CD44 antibody [MEM-85] (Biotin), followed by Streptavidin (APC).

Clone References

Umbilical cord mesenchymal stem cells suppress host rejection: the role of the glycocalyx.

ICC/IF / Human

Coulson-Thomas VJ et al.
J Biol Chem.,  (2014)

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Organization and regulation of intracellular plasma membrane-connected HIV-1 assembly compartments in macrophages.

ICC/IF / Human

Mlcochova P et al.
BMC Biol.,  (2013)

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Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules.

Stefanová I et al.
Immunogenetics.,  (1989)

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Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.

Bazil V et al.
Folia Biol (Praha).,  (1989)

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