ARG22713
anti-CD41 + CD61 antibody [CO.35E4]
anti-CD41 + CD61 antibody [CO.35E4] for ELISA,Flow cytometry,Immunoprecipitation and Bovine,Cat,Dog,Goat,Horse,Rabbit,Sheep
Overview
| Product Description | Mouse Monoclonal antibody [CO.35E4] recognizes CD41 + CD61 This antibody recognizes ruminant CD41/CD61, also known as GpIIb/IIIa. CD41/CD61 is a calcium-complex dependent heterodimer and is expressed almost exclusively on platelets and on their precursors, the megakaryocytes. This complex is an activation-dependent receptor for RGD (Arg-Gly-Asp)-containing glycoproteins such as fibrinogen, fibronectin, von Willebrand factor, thrombospondin and vitronectin. It plays a central role in platelet aggregation and haemostasis. |
|---|---|
| Tested Reactivity | Bov, Cat, Dog, Goat, Hrs, Rb, Sheep |
| Tested Application | ELISA, FACS, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CO.35E4 |
| Isotype | IgG1 |
| Target Name | CD41 + CD61 |
| Antigen Species | Sheep |
| Immunogen | Sheep leucocytes |
| Conjugation | Un-conjugated |
| Alternate Names | GTA; GT; GPalpha IIb; PPP1R93; CD41; BDPLT2; BDPLT16; GP2B; Integrin alpha-IIb; GPIIb; Platelet membrane glycoprotein IIb; HPA3; CD antigen CD41; CD41B |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | ITGA2B |
|---|---|
| Gene Full Name | integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) |
| Background | ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008] |
| Function | Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProt] |
| Calculated MW | 113 kDa |
Images (1) Click the Picture to Zoom In
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ARG22713 anti-CD41 + CD61 antibody [CO.35E4] FACS image
Flow Cytometry: Sheep peripheral blood platelets stained with ARG22713 anti-CD41 + CD61 antibody [CO.35E4].
