ARG23340

anti-CD40 antibody [B-B20] (Biotin)

anti-CD40 antibody [B-B20] (Biotin) for Flow cytometry and Human

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [B-B20] recognizes CD40
Tested Reactivity Hu
Tested Application FACS
Specificity This antibody recognizes the Bp50 antigen, a 48 kDa protein.
Host Mouse
Clonality Monoclonal
Clone B-B20
Isotype IgG1
Target Name CD40
Antigen Species Human
Immunogen Raji cell line
Conjugation Biotin
Alternate Names CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
Application Note FACS: Use 10 µl to label 10^6 cells or 100 µl of whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS, 0.1% Sodium azide and 1% BSA.
Preservative 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 958 Human CD40

Swiss-port # P25942 Human Tumor necrosis factor receptor superfamily member 5

Gene Symbol CD40
Gene Full Name CD40 molecule, TNF receptor superfamily member 5
Background This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Function Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa