ARG22902

anti-CD40 antibody [3/23] (low endotoxin)

anti-CD40 antibody [3/23] (low endotoxin) for ELISA,Flow cytometry,Functional study,IHC-Frozen sections and Mouse

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [3/23] recognizes CD40
Rat anti Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40.Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Tested Reactivity Ms
Tested Application ELISA, FACS, FuncSt, IHC-Fr
Host Rat
Clonality Monoclonal
Clone 3/23
Isotype IgG2a
Target Name CD40
Antigen Species Mouse
Immunogen Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
Conjugation Un-conjugated
Alternate Names CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACSNeat - 1:5
FuncStAssay-dependent
IHC-FrAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note Low endotoxin
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21939 Mouse CD40

Swiss-port # P27512 Mouse Tumor necrosis factor receptor superfamily member 5

Gene Symbol Cd40
Gene Full Name CD40 antigen
Background This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Function Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa

Images (1) Click the Picture to Zoom In

  • ARG22902 anti-CD40 antibody [3/23] (low endotoxin) FACS image

    Flow Cytometry: Mouse splenocytes stained with ARG22902 anti-CD40 antibody [3/23] (low endotoxin).