ARG43904
anti-CD3G antibody
anti-CD3G antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD3G |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD3G |
| Antigen Species | Human |
| Immunogen | Human CD3G recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | T-cell surface glycoprotein CD3 gamma chain |
| Alternate Names | CD3G; CD3 Gamma Subunit Of T-Cell Receptor Complex; T-Cell Surface Glycoprotein CD3 Gamma Chain; CD3-GAMMA; CD3GAMMA; CD3g Antigen, Gamma Polypeptide (TiT3 Complex); CD3g Molecule, Gamma (CD3-TCR Complex); T-Cell Receptor T3 Gamma Chain; T3G; T-Cell Antigen Receptor Complex, Gamma Subunit Of T3; CD3g Molecule, Epsilon (CD3-TCR Complex); CD3g Molecule; CD3g Antigen; IMD17 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links |
Swiss-port # P09693 Human T-cell surface glycoprotein CD3 gamma chain |
|---|---|
| Gene Symbol | CD3G |
| Gene Full Name | CD3 Gamma Subunit Of T-Cell Receptor Complex |
| Background | The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008] |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways |
| Cellular Localization | Membrane |
| Calculated MW | 21 kDa |
| PTM | Disulfide bond, Glycoprotein, Phosphoprotein |
Images (3) Click the Picture to Zoom In
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ARG43904 anti-CD3G antibody ICC/IF image
Immunofluorescence: JK cells stained with ARG43904 anti-CD3G antibody at 5 μg/ml dilution.
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ARG43904 anti-CD3G antibody WB image
Western blot: Jurkat stained with ARG43904 anti-CD3G antibody at 0.5 μg/mL dilution.
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ARG43904 anti-CD3G antibody FACS image
Flow Cytometry: JK cells stained with ARG43904 anti-CD3G antibody (blue) at 1 μg/1x10^6 cells dilution.
