ARG62827
anti-CD361 / EVI2B antibody [MEM-216]
anti-CD361 / EVI2B antibody [MEM-216] for Flow cytometry,Immunoprecipitation and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-216] recognizes CD361 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP |
| Specificity | The clone MEM-216 recognizes CD361 / EVI2B, almost uncharacterized type I transmembrane protein with broad leukocyte expression, mostly in myeloid and B cells. HLDA IX.; WS Code 263 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-216 |
| Isotype | IgG1 |
| Target Name | CD361 / EVI2B |
| Antigen Species | Human |
| Immunogen | Raji cells_x000D_ |
| Conjugation | Un-conjugated |
| Alternate Names | CD361; EVDB; EVI-2B; CD antigen CD361; Protein EVI2B; Ecotropic viral integration site 2B protein homolog; D17S376 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | FACS: Positive control: Raji, Daudi, HL-60 and peripheral blood lymphcocytes (strongly positive on CD19+ cells). Negative control: Jurkat and U-937 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EVI2B |
| Gene Full Name | ecotropic viral integration site 2B |
| Background | CD361, also known as EVI2B (Ecotropic Viral Integration site 2B) or EVDB, is a poorly characterized type I transmembrane protein, expressed from one of three genes embedded in intron 27b of the neurofibromatosis type 1 (NF1) gene. The DNA strand that is transcribed to produce CD361 is the complementary one to the strand encoding NF1. Murine homolog to human CD361 is associated with ecotropic viral insertions, which have been implicated in the expression of murine myeloid leukemias. CD361 has been also reported to be involved in melanocyte and keratinocyte differentiation. However, it is expressed mainly in peripheral blood and bone marrow. |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 49 kDa |
Images (1) Click the Picture to Zoom In
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ARG62827 anti-CD361 / EVI2B antibody [MEM-216] FACS image
Flow Cytometry: CD19+ peripheral blood leukocytes stained with ARG62827 anti-CD361 / EVI2B antibody [MEM-216].
