ARG65437
anti-CD35 / CR1 antibody [E11]
anti-CD35 / CR1 antibody [E11] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Primates
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [E11] recognizes CD35 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone E11 recognizes CD35 (CR1), a type I glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | E11 |
| Isotype | IgG1 |
| Target Name | CD35 / CR1 |
| Immunogen | Acute monocytic leukemia cells and normal blood monocytes |
| Conjugation | Un-conjugated |
| Alternate Names | C3b/C4b receptor; C4BR; CD antigen CD35; KN; CD35; C3BR; Complement receptor type 1 |
Application Instructions
| Application Suggestion |
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| Application Note | Immunohistochemistry (paraffin sections): heat mediated antigen retrieval is require before staining Immunohistochemistry (frozen sections): acetone fixation is recommended * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CR1 |
| Gene Full Name | complement component (3b/4b) receptor 1 (Knops blood group) |
| Background | CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production. |
| Function | Mediates cellular binding of particles and immune complexes that have activated complement. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 224 kDa |
Clone References
Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor.
ELISA /
Effect of B-cell depletion on coreceptor switching in R5 simian-human immunodeficiency virus infection of rhesus macaques.
IHC / Human
Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum.
ELISA / Human
