ARG44058
anti-CD34 antibody [QBEND/10] (PerCP-Cyanine 5.5)
anti-CD34 antibody [QBEND/10] (PerCP-Cyanine 5.5) for Flow cytometry and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Overview
| Product Description | PerCP-Cyanine 5.5-conjugated Mouse Monoclonal antibody [QBEND/10] recognizes CD34 (Endothelial Cell Marker) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND/10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. The clone QBEND/10 recognises the Class II epitope on CD34. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | QBEND/10 |
| Isotype | IgG1 |
| Target Name | CD34 |
| Antigen Species | Human |
| Immunogen | Human endothelial vesicles |
| Conjugation | PerCP-Cyanine 5.5 |
| Alternate Names | Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | The purified antibody is conjugated with PerCP-Cyanine 5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34 |
|---|---|
| Gene Symbol | CD34 |
| Gene Full Name | CD34 molecule |
| Background | CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Function | CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody |
| Calculated MW | 41 kDa |
| PTM | Highly glycosylated. Phosphorylated on serine residues by PKC. |
Images (1) Click the Picture to Zoom In
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ARG44058 anti-CD34 antibody [QBEND/10] (PerCP-Cyanine 5.5) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG44058 anti-CD34 antibody [QBEND/10] (PerCP-Cyanine 5.5) at 10 μl / 10^6 cells dilution.
