ARG62822

anti-CD34 antibody [QBEND/10] (FITC)

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100 tests

389.00

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Datasheet
Publication

Overview

Product Description	FITC-conjugated Mouse Monoclonal antibody [QBEND/10] recognizes CD34 (Endothelial Cell Marker)
Tested Reactivity	Hu, NHuPrm
Species Does Not React With	Rat, Bov, Dog, Sheep
Tested Application	FACS
Specificity	The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND/10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. The clone QBEND/10 recognises the Class II epitope on CD34.
Host	Mouse
Clonality	Monoclonal
Clone	QBEND/10
Isotype	IgG1, kappa
Target Name	CD34
Antigen Species	Human
Immunogen	Human endothelial vesicles
Conjugation	FITC
Alternate Names	Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	20 µl / 10^6 cells

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification Note	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative	15 mM Sodium azide
Stabilizer	0.2% (w/v) high-grade protease free BSA
Storage Instruction	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 947 Human CD34 Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34
Gene Symbol	CD34
Gene Full Name	CD34 molecule
Background	CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function	CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Calculated MW	41 kDa
PTM	Highly glycosylated. Phosphorylated on serine residues by PKC.

Images (3) Click the Picture to Zoom In

ARG62822 anti-CD34 antibody [QBEND/10] (FITC) FACS image

Flow Cytometry: Human peripheral whole blood stained with ARG62822 anti-CD34 antibody [QBEND/10] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).
ARG62822 anti-CD34 antibody [QBEND/10] (FITC) FACS image

Flow Cytometry: Separation of human CD34 positive stem cells (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62822 anti-CD34 antibody [QBEND/10] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).
ARG62822 anti-CD34 antibody [QBEND/10] (FITC) FACS image

Flow Cytometry: Human peripheral whole blood showing CD34 positive stem cells (red). Cells were stained with ARG62822 anti-CD34 antibody [QBEND/10] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).

Clone References

c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2α and promotes tumor angiogenesis and metastasis by upregulating FGF2.

Xue G et al.
Oncogene., (2015)

Circulating hematopoietic stem and progenitor cells are myeloid-biased in cancer patients.

Wu WC et al.
Proc Natl Acad Sci U S A., (2014)

Origin of the vasculature supporting growth of primary patient tumor xenografts.

IHC-P, IHC-Fr / Human

Hylander BL et al.
J Transl Med., (2013)

Cyclic tensile stretch modulates osteogenic differentiation of adipose-derived stem cells via the BMP-2 pathway.

ICC/IF / Rat

Yang X et al.
Arch Med Sci., (2010)

Overexpression of thrombospondin-1 in stromal myofibroblasts is associated with tumor growth and nodal metastasis in gastric carcinoma.

IHC / Human

Lin XD et al.
J Surg Oncol., (2012)

Evidence of intra-hepatic vascular proliferation remodeling early after cure in experimental schistosomiasis mansoni: an immunohistochemical descriptive study.

IHC / Mouse

Abdel Fattah NS et al.
Exp Parasitol., (2012)

Genetic predisposition directs breast cancer phenotype by dictating progenitor cell fate.

FACS / Human

Proia TA et al.
Cell Stem Cell., (2011)

Fibronectin promotes the phorbol 12-myristate 13-acetate-induced macrophage differentiation in myeloid leukemia cells.

ICC/IF / Human

Esendagli G et al.
Int J Hematol., (2009)

Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo.

FACS / Rabbit

Gu S et al.
Mol Vis., (2009)

Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.

IHC / Rat

Esendagli G et al.
Lab Anim., (2009)

Optimal perfusion of an intact ovary as a prerequisite for successful ovarian cryopreservation.

IHC / Bovine

Gerritse R et al.
Hum Reprod., (2008)

1,1-Bis(3'-indolyl)-1-(p-biphenyl)methane inhibits basal-like breast cancer growth in athymic nude mice.

ICC/IF / Mouse

Su Y et al.
Breast Cancer Res., (2007)

Recurrent solitary fibrous tumour in the cerebellopontine angle.

IHC / Dog

Bikmaz K et al.
J Clin Neurosci., (2005)