ARG22956

anti-CD34 antibody [MEC14.7]

Datasheet

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100 μg

379.00

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Datasheet

Overview

Product Description	Rat Monoclonal antibody [MEC14.7] recognizes CD34 Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
Tested Reactivity	Ms
Tested Application	CyTOF®-candidate, FACS, ICC/IF, IHC-P, IP
Host	Rat
Clonality	Monoclonal
Clone	MEC14.7
Isotype	IgG2a
Target Name	CD34
Antigen Species	Mouse
Immunogen	T-end.1, a pMT transformed endothelial cell line.
Conjugation	Un-conjugated
Alternate Names	Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion

Tested Application	Dilution
CyTOF®-candidate	Assay-dependent
FACS	1:10 - 1:20
ICC/IF	Assay-dependent
IHC-P	1:20 - 1:200
IP	2 - 10 µg/ml

Application Note

FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS and 0.09% Sodium azide
Preservative	0.09% Sodium azide
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12490 Mouse CD34 Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34
Gene Symbol	Cd34
Gene Full Name	CD34 antigen
Background	CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function	CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Highlight	Related products: CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: CyTOF-candidate Antibodies
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Calculated MW	41 kDa
PTM	Highly glycosylated. Phosphorylated on serine residues by PKC.