ARG42236

anti-CD34 antibody

anti-CD34 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Dog

Overview

Product Description Rabbit Polyclonal antibody recognizes CD34
Tested Reactivity Hu, Ms, Rat, Dog
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD34
Antigen Species Human
Immunogen Synthetic peptide of Human CD34.
Conjugation Un-conjugated
Alternate Names Hematopoietic progenitor cell antigen CD34; CD antigen CD34

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NIH/3T3
Observed Size ~ 120 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12490 Mouse CD34

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34

Gene Symbol CD34
Gene Full Name CD34 molecule
Background The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 41 kDa
PTM Highly glycosylated.

Phosphorylated on serine residues by PKC. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG42236 anti-CD34 antibody ICC/IF image

    Immunofluorescence: HUVEC cells stained with ARG42236 anti-CD34 antibody.

  • ARG42236 anti-CD34 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue stained with ARG42236 anti-CD34 antibody.

  • ARG42236 anti-CD34 antibody WB image

    Western blot: NIH/3T3 cell lysate stained with ARG42236 anti-CD34 antibody.