ARG42236
anti-CD34 antibody
anti-CD34 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Dog
Overview
Product Description | Rabbit Polyclonal antibody recognizes CD34 |
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Tested Reactivity | Hu, Ms, Rat, Dog |
Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | CD34 |
Antigen Species | Human |
Immunogen | Synthetic peptide of Human CD34. |
Conjugation | Un-conjugated |
Alternate Names | Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
Positive Control | NIH/3T3 | ||||||||||||
Observed Size | ~ 120 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34 Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34 |
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Gene Symbol | CD34 |
Gene Full Name | CD34 molecule |
Background | The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
Function | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt] |
Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
Calculated MW | 41 kDa |
PTM | Highly glycosylated. Phosphorylated on serine residues by PKC. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG42236 anti-CD34 antibody ICC/IF image
Immunofluorescence: HUVEC cells stained with ARG42236 anti-CD34 antibody.
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ARG42236 anti-CD34 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue stained with ARG42236 anti-CD34 antibody.
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ARG42236 anti-CD34 antibody WB image
Western blot: NIH/3T3 cell lysate stained with ARG42236 anti-CD34 antibody.