ARG65718
anti-CD34 antibody [581]
anti-CD34 antibody [581] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections and Human,Primates
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [581] recognizes CD34 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | CyTOF®-candidate, FACS, IHC-Fr |
| Specificity | This antibody reacts with CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 581 |
| Isotype | IgG1 |
| Target Name | CD34 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34 |
|---|---|
| Gene Symbol | CD34 |
| Gene Full Name | CD34 molecule |
| Background | CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Function | CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt] |
| Highlight | Related products: CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody |
| Calculated MW | 41 kDa |
| PTM | Highly glycosylated. Phosphorylated on serine residues by PKC. |
Images (1) Click the Picture to Zoom In
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ARG65718 anti-CD34 antibody [581] FACS image
Flow Cytometry: Human peripheral blood stained with ARG65718 anti-CD34 antibody [581], followed by incubation with APC labelled Goat anti-Mouse secondary antibody. CD34+ cells (blue); Leukocytes (red).
