ARG23573
anti-CD32B antibody [AT130-5R]
anti-CD32B antibody [AT130-5R] for IHC-Frozen sections and Mouse
Overview
| Product Description | Rat Monoclonal antibody [AT130-5R] recognizes CD32B. Rat anti Mouse CD32B, clone AT130-5R, recognizes mouse CD32B also known as FcRIIB. CD32B (FcRIIB) is a low affinity receptor for IgG1, IgG2a and IgG2b type antibodies. Unlike other FcRs, the intracellular domain of CD32 contains a inhibitory signalling motif (ITIM) that can antagonize signal transduction mediated through other activatory receptors. FcRIIB is expressed on all myeloid populations and on B-cells. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | IHC-Fr |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | AT130-5R |
| Isotype | IgG2b |
| Target Name | CD32B |
| Antigen Species | Mouse |
| Immunogen | Mouse CD32-Rat CD4 fusion protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | TRIS buffered saline and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08101 Mouse Low affinity immunoglobulin gamma Fc region receptor II |
|---|---|
| Gene Symbol | FCGR2B |
| Gene Full Name | Fc fragment of IgG, low affinity IIb, receptor (CD32) |
| Background | The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Function | Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG23573 anti-CD32B antibody [AT130-5R] IHC-Fr image
Immunohistochemistry: Acetone-fixed frozen sections of BALB/c WT and CD32B -/- Mouse spleen and liver (10 µm) stained with ARG23573 anti-CD32B antibody [AT130-5R] at 5 µg/ml followed by an anti-Rat IgG HRP polymer and counterstained with Haematoxylin.
