ARG63774
anti-CD32B antibody
anti-CD32B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CD32B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD32B |
| Antigen Species | Human |
| Immunogen | C-PDALEEPDDQNRI |
| Conjugation | Un-conjugated |
| Alternate Names | Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P31994 Human Low affinity immunoglobulin gamma Fc region receptor II-b |
|---|---|
| Background | The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 36 kDa |
| PTM | Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. |
Images (5) Click the Picture to Zoom In
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ARG63774 anti-CD32B antibody WB image
Western blot: K562 lysate (35 µg protein in RIPA buffer) stained with ARG63774 anti-CD32B antibody at 0.3 µg/ml dilution.
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ARG63774 anti-CD32B antibody WB image
Western blot: 1). Mock transfection; 2) CD32 (RC211982) expressing plasmid transfected HEK293 cell lysate standed with ARG63774 anti-CD32B antibody.
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ARG63774 anti-CD32B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63774 anti-CD32B antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63774 anti-CD32B antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63774 anti-CD32B antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63774 anti-CD32B antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG63774 anti-CD32B antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
