ARG23474
anti-CD32 antibody [CCG36] (PE)
anti-CD32 antibody [CCG36] (PE) for Flow cytometry and Bovine,Sheep
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [CCG36] recognizes CD32 Mouse anti Bovine CD32 antibody, clone CCG36 recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils. CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009. Clone CCG36 also recognizes ovine CD32. |
|---|---|
| Tested Reactivity | Bov, Sheep |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CCG36 |
| Isotype | IgG1 |
| Target Name | CD32 |
| Antigen Species | Bovine |
| Immunogen | Bovine FcγRII-transfected COS7 cells. |
| Conjugation | PE |
| Alternate Names | Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q28110 Bovine Low affinity immunoglobulin gamma Fc region receptor II |
|---|---|
| Gene Symbol | FCGR2B |
| Gene Full Name | Fc fragment of IgG, low affinity IIb, receptor (CD32) |
| Background | The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Function | Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG23474 anti-CD32 antibody [CCG36] (PE) FACS image
Flow Cytometry: Bovine peripheral blood lymphocytes stained with ARG23474 anti-CD32 antibody [CCG36] (PE).
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ARG23474 anti-CD32 antibody [CCG36] (PE) FACS image
Flow Cytometry: Figure A. FITC conjugated Mouse anti Bovine CD282 and PE-conjugated Mouse IgG1 isotype control. Figure B. FITC conjugated Mouse anti Bovine CD282 and ARG23474 anti-CD32 antibody [CCG36] (PE). All experiments performed on red cell lysed Bovine blood gated on mononuclear cells.
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ARG23474 anti-CD32 antibody [CCG36] (PE) FACS image
Flow Cytometry: Figure A. ARG23474 anti-CD32 antibody [CCG36] (PE) and FITC conjugated HuCAL isotype control. Figure B. ARG23474 anti-CD32 antibody [CCG36] (PE) and FITC conjugated Mouse anti Bovine CD282. All experiments performed on red cell lysed Bovine blood gated on mononuclear cells.
