ARG54201
anti-CD32 antibody [3D3] (PE)
anti-CD32 antibody [3D3] (PE) for Flow cytometry and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [3D3] recognizes CD32 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody 3D3 recognizes CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3D3 |
| Isotype | IgG1 |
| Target Name | CD32 |
| Antigen Species | Human |
| Immunogen | purified glycosylated recombinant human FcgammaRIIa2 |
| Conjugation | PE |
| Alternate Names | Fc-gamma RII-a; CD antigen CD32; FcGR; IgG Fc receptor II-a; FCGR2A1; CD32A; FCGR2; Low affinity immunoglobulin gamma Fc region receptor II-a; FcRII-a; Fc-gamma-RIIa; FCG2; IGFR2; CD32; CDw32 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12318 Human Low affinity immunoglobulin gamma Fc region receptor II-a |
|---|---|
| Gene Symbol | FCGR2A |
| Gene Full Name | Fc fragment of IgG, low affinity IIa, receptor (CD32) |
| Background | CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes). |
| Function | Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 35 kDa |
| PTM | Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. |
Images (2) Click the Picture to Zoom In
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ARG54201 anti-CD32 antibody [3D3] (PE) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG54201 anti-CD32 antibody [3D3] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
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ARG54201 anti-CD32 antibody [3D3] (PE) FACS image
Flow Cytometry: Separation of human CD32 positive lymphocytes (red-filled) from CD32 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG54201 anti-CD32 antibody [3D3] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
