ARG65378

anti-CD314 / NKG2D antibody [1D11]

anti-CD314 / NKG2D antibody [1D11] for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [1D11] recognizes CD314 / NKG2D
Tested Reactivity Hu
Tested Application FACS, FuncSt, IHC-Fr, IP
Specificity The clone 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.
Host Mouse
Clonality Monoclonal
Clone 1D11
Isotype IgG1
Target Name CD314 / NKG2D
Immunogen NKL cell line
Conjugation Un-conjugated
Alternate Names NKG2-D-activating NK receptor; CD antigen CD314; D6H12S2489E; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D; Killer cell lectin-like receptor subfamily K member 1; Nkg2d

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note Functional studies: Blocking of ligand binding.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22914 Human KLRK1

Swiss-port # P26718 Human NKG2-D type II integral membrane protein

Gene Symbol KLRK1
Gene Full Name killer cell lectin-like receptor subfamily K, member 1
Background CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.
Function Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. [UniProt]
Research Area Immune System antibody
Calculated MW 25 kDa

Images (2) Click the Picture to Zoom In

  • ARG65378 anti-CD314 / NKG2D antibody [1D11] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG65378 anti-CD314 / NKG2D antibody [1D11] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG65378 anti-CD314 / NKG2D antibody [1D11] FACS image

    Flow Cytometry: Separation of human CD314 / NKG2D positive lymphocytes (red-filled) from CD314 / NKG2D negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65378 anti-CD314 / NKG2D antibody [1D11] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

Clone References

In vitro Natural Killer Cell Immunotherapy for Medulloblastoma.

FACS / Human

Fernández L et al.
Front Oncol.,  (2013)

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Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patients.

FACS / Human

Reiners KS et al.
Mol Ther.,  (2013)

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Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells.

FuncSt / Human

Abeyweera TP et al.
J Cell Biol.,  (2011)

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Genome-wide association study in alopecia areata implicates both innate and adaptive immunity.

IHC-Fr / Human

Petukhova L et al.
Nature.,  (2010)

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