ARG62811
anti-CD300a + CD300c antibody [MEM-260]
anti-CD300a + CD300c antibody [MEM-260] for Flow cytometry,Western blot and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-260] recognizes CD300a + CD300c |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Specificity | The clone MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-260 |
| Isotype | IgG1 |
| Target Name | CD300a + CD300c |
| Antigen Species | Human |
| Immunogen | HPB human acute lymphoid leukemia cell line |
| Conjugation | Un-conjugated |
| Alternate Names | CMRF-35-H9; Inhibitory receptor protein 60; NK inhibitory receptor; CMRF35-H; CMRF35H9; CD antigen CD300a; IRC1/IRC2; IGSF12; CMRF35H; IRp60; IgSF12; CMRF35-like molecule 8; CMRF35-H9; CMRF-35H; CD300 antigen-like family member A; CLM-8; IRC1; IRC2; Immunoglobulin superfamily member 12 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: Positive control: HPB. Negative control: Jurkat and Raji. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD300A |
| Gene Full Name | CD300a molecule |
| Background | CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity. |
| Function | Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated on tyrosine. N-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG62811 anti-CD300a + CD300c antibody [MEM-260] FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG62811 anti-CD300a + CD300c antibody [MEM-260], followed by incubation with APC labelled Goat anti-Mouse secondary antibody.
