ARG20469
anti-CD284 / TLR4 antibody
anti-CD284 / TLR4 antibody for Western blot and Rat
Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD284 / TLR4 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD284 / TLR4 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around 819 aa of Rat TLR4 protein (NP_062051.1) |
| Conjugation | Un-conjugated |
| Alternate Names | CD284; CD antigen CD284; ARMD10; hToll; TLR-4; TOLL; Toll-like receptor 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 30% Glycerol, 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Tlr4 |
| Gene Full Name | toll-like receptor 4 |
| Background | TLR4 (Toll-like receptor 4) is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB. |
| Highlight | Related products: TLR4 antibodies; TLR4 ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: Detecting exosomal HMGB1 for ICD research |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 96 kDa |
| PTM | N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. |
Images (1) Click the Picture to Zoom In
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ARG20469 anti-CD284 / TLR4 antibody WB image
Western blot: Rat kidney tissue lysate stained with ARG20469 anti-CD284 / TLR4 antibody.
