ARG65473

anti-CD28 antibody [JJ319] (PE)

anti-CD28 antibody [JJ319] (PE) for Flow cytometry and Rat

Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [JJ319] recognizes CD28
Tested Reactivity Rat
Tested Application FACS
Specificity The clone JJ319 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
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Host Mouse
Clonality Monoclonal
Clone JJ319
Isotype IgG1
Target Name CD28
Antigen Species Rat
Immunogen Mouse A20J B lymphoma cells transfected with rat CD28
Conjugation PE
Alternate Names Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25660 Rat CD28

Swiss-port # P31042 Rat T-cell-specific surface glycoprotein CD28

Gene Symbol Cd28
Gene Full Name Cd28 molecule
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. Its analog CTLA-4, expressed on the surface of activated T cells, has higher affinity to CD80 and CD86 than CD28 and downregulates T cell activity.
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Calculated MW 25 kDa
PTM CD40LG induces tyrosine phosphorylation of isoform 3.

Clone References

Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ester.

Tacke M et al.
J Immunol.,  (1995)

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