ARG65471
anti-CD28 antibody [JJ319]
anti-CD28 antibody [JJ319] for Flow cytometry,Functional study,Immunoprecipitation and Rat
Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [JJ319] recognizes CD28 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS, FuncSt, IP |
| Specificity | The clone JJ319 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. _x000D_ |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | JJ319 |
| Isotype | IgG1 |
| Target Name | CD28 |
| Antigen Species | Rat |
| Immunogen | Mouse A20J B lymphoma cells transfected with rat CD28 |
| Conjugation | Un-conjugated |
| Alternate Names | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: In vitro costimulation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P31042 Rat T-cell-specific surface glycoprotein CD28 |
|---|---|
| Gene Symbol | Cd28 |
| Gene Full Name | Cd28 molecule |
| Background | CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. Its analog CTLA-4, expressed on the surface of activated T cells, has higher affinity to CD80 and CD86 than CD28 and downregulates T cell activity. |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 25 kDa |
| PTM | CD40LG induces tyrosine phosphorylation of isoform 3. |
Images (1) Click the Picture to Zoom In
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ARG65471 anti-CD28 antibody [JJ319] FACS image
Flow Cytometry: Rat splenocytes suspension stained with ARG65471 anti-CD28 antibody [JJ319] at 4 μg/ml dilution.
Clone References
Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ester.
