ARG65638
anti-CD28 antibody
anti-CD28 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CD28 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Target Name | CD28 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the center region of Human CD28 (SQQLQVYSKTGFNCD) |
| Conjugation | Un-conjugated |
| Alternate Names | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28 |
|---|---|
| Gene Symbol | CD28 |
| Gene Full Name | CD28 molecule |
| Background | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. |
| Research Area | Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| Calculated MW | 25 kDa |
| PTM | CD40LG induces tyrosine phosphorylation of isoform 3. |
Images (2) Click the Picture to Zoom In
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ARG65638 anti-CD28 antibody WB image
Western blot: 35 μg of Human Tonsil lysate stained with ARG65638 anti-CD28 antibody at 0.3 μg/ml dilution (1h incubation).
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ARG65638 anti-CD28 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lymph node tissue. Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65638 anti-CD28 antibody at 2 µg/ml dilution followed by HRP-staining.
