ARG65637

anti-CD28 antibody

anti-CD28 antibody for Western blot and Mouse

Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody

Overview

Product Description Goat Polyclonal antibody recognizes CD28
Tested Reactivity Ms
Tested Application WB
Host Goat
Clonality Polyclonal
Target Name CD28
Antigen Species Mouse
Immunogen Synthetic peptide around the center region of Mouse CD28 (KEKHLCHTQSSPK)
Conjugation Un-conjugated
Alternate Names Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12487 Mouse CD28

Swiss-port # P31041 Mouse T-cell-specific surface glycoprotein CD28

Gene Symbol Cd28
Gene Full Name CD28 antigen
Background The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Research Area Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Calculated MW 25 kDa 
PTM CD40LG induces tyrosine phosphorylation of isoform 3.

Images (1) Click the Picture to Zoom In

  • ARG65637 anti-CD28 antibody WB image

    Western blot: 35 μg of Mouse Thymus lysate stained with ARG65637 anti-CD28 antibody at 0.3 μg/ml dilution (1h incubation).