ARG59551
anti-CD27 antibody
anti-CD27 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD27 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD27 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A20-R191 of Human CD27. |
| Conjugation | Un-conjugated |
| Alternate Names | T-cell activation antigen CD27; Tp55; CD antigen CD27; CD27 antigen; S152. LPFS2; T14; S152; Tumor necrosis factor receptor superfamily member 7; CD27L receptor; TNFRSF7 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD27 |
| Gene Full Name | CD27 molecule |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008] |
| Function | Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 29 kDa |
| PTM | Phosphorylated. O-glycosylated with core 1 or possibly core 8 glycans. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG59551 anti-CD27 antibody ICC/IF image
Immunofluorescence: K562 cells were blocked with 10% goat serum and then stained with ARG59551 anti-CD27 antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59551 anti-CD27 antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and stained with ARG59551 anti-CD27 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59551 anti-CD27 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil stained with ARG59551 anti-CD27 antibody at 1 µg/ml dilution.
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ARG59551 anti-CD27 antibody WB image
Western blot: HeLa whole cell lysate stained with ARG59551 anti-CD27 antibody at 0.5 µg/ml dilution.
