ARG65468
anti-CD266 / TWEAK R antibody [ITEM-4]
anti-CD266 / TWEAK R antibody [ITEM-4] for Flow cytometry,Functional study,IHC-Frozen sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [ITEM-4] recognizes CD266 / TWEAK R |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, FuncSt, IHC-Fr, WB |
| Specificity | The clone ITEM-4 recognizes CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ITEM-4 |
| Isotype | IgG2b |
| Target Name | CD266 / TWEAK R |
| Antigen Species | Human |
| Immunogen | human CD266-transfected P815 cells |
| Conjugation | Un-conjugated |
| Alternate Names | Tumor necrosis factor receptor superfamily member 12A; FGF-inducible 14; CD266; Fibroblast growth factor-inducible immediate-early response protein 14; TWEAKR; TweakR; CD antigen CD266; Tweak-receptor; FN14 |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Blocking. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9CR75 Mouse Tumor necrosis factor receptor superfamily member 12A Swiss-port # Q9NP84 Human Tumor necrosis factor receptor superfamily member 12A |
|---|---|
| Gene Symbol | TNFRSF12A |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 12A |
| Background | CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential. |
| Function | Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 14 kDa |
Images (2) Click the Picture to Zoom In
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ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] FACS image
Flow Cytometry: HUVEC cells stained with ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] FACS image
Flow Cytometry: Separation of stained HUVEC cells (red-filled) from unstained HUVEC cells (black-dashed). Cells were stained with ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
TWEAK mediates signal transduction and differentiation of RAW264.7 cells in the absence of Fn14/TweakR. Evidence for a second TWEAK receptor.
