ARG62792

anti-CD264 / TRAIL R4 antibody [TRAIL-R4-01]

anti-CD264 / TRAIL R4 antibody [TRAIL-R4-01] for Flow cytometry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [TRAIL-R4-01] recognizes CD264 / TRAIL R4
Tested Reactivity Hu
Tested Application FACS
Specificity The clone TRAIL-R4-01 reacts with TRAIL-R4, a 42 kDa transmembrane protein expressed on various blood cells.
Host Mouse
Clonality Monoclonal
Clone TRAIL-R4-01
Isotype IgG1
Target Name CD264 / TRAIL R4
Immunogen TRAIL-R4 (aa 1-210) - hIgGhc fusion protein
Conjugation Un-conjugated
Alternate Names Tumor necrosis factor receptor superfamily member 10D; CD264; DCR2; CD antigen CD264; DcR2; Decoy receptor 2; TNF-related apoptosis-inducing ligand receptor 4; TRUNDD; TRAILR4; TRAIL receptor with a truncated death domain; TRAIL receptor 4; TRAIL-R4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8793 Human TNFRSF10D

Swiss-port # Q9UBN6 Human Tumor necrosis factor receptor superfamily member 10D

Gene Symbol TNFRSF10D
Gene Full Name tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
Background TRAIL-R4 (CD264, TR4, DcR2, TRUNDD), expressed mainly on CD8+ and NK cells, belongs to receptors of TRAIL, a TNF-like membrane toxic protein that induces apoptosis in many tumour cells, but not in normal cells. TRAIL-R4, however, contains partially truncated death domain, thus it is unable to induce apoptosis and serves as a negative regulator of apoptotic signaling by impairment death-inducing signaling complex (DISC) processing. TRAIL-R4 interacts with death receptor 5 (DR5) in the native DISC in a TRAIL-dependent manner and prevents its corecruitment with death receptor 4 (DR4).
Function Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody
Calculated MW 42 kDa

Images (1) Click the Picture to Zoom In

  • ARG62792 anti-CD264 / TRAIL R4 antibody [TRAIL-R4-01] FACS image

    Flow Cytometry: Separation of CD264 transfected HEK-293 cells (red-filled) from non-transfected HEK-293 cells (black-dashed). Human peripheral whole blood stained with ARG62792 anti-CD264 / TRAIL R4 antibody [TRAIL-R4-01] at 0.33 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.