ARG62791
anti-CD263 / TRAIL R3 antibody [TRAIL-R3-02]
anti-CD263 / TRAIL R3 antibody [TRAIL-R3-02] for Flow cytometry and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [TRAIL-R3-02] recognizes CD263 / TRAIL R3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone TRAIL-R3-02 reacts with TRAIL-R3, a 35 kDa GPI-anchored extracellular membrane protein expressed mainly on neutrophils. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TRAIL-R3-02 |
| Isotype | IgG1 |
| Target Name | CD263 / TRAIL R3 |
| Immunogen | TRAIL-R3 (aa 1-280) - hIgGhc fusion protein |
| Conjugation | Un-conjugated |
| Alternate Names | Lymphocyte inhibitor of TRAIL; Antagonist decoy receptor for TRAIL/Apo-2L; TNF-related apoptosis-inducing ligand receptor 3; DCR1; TRID; CD antigen CD263; Tumor necrosis factor receptor superfamily member 10C; CD263; Decoy TRAIL receptor without death domain; LIT; Decoy receptor 1; DcR1; DCR1-TNFR; TRAIL-R3; TRAIL receptor 3; TRAILR3; TRAIL receptor without an intracellular domain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O14798 Human Tumor necrosis factor receptor superfamily member 10C |
|---|---|
| Gene Symbol | TNFRSF10C |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain |
| Background | TRAIL-R3 (CD263, TR3, DcR1, LIT, TRID), expressed mainly on neutrophils, belongs to receptors of TRAIL, a TNF-like membrane cytotoxic protein that induces apoptosis in many tumour cells, but not in normal cells. TRAIL-R3, however, is a GPI-anchored protein that lacks cytoplasmic death domain, thus it is unable to induce apoptosis and serves as a negative regulator of apoptotic signaling by competing for binding of TRAIL with death receptor 5 (DR5). |
| Function | Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody |
| Calculated MW | 27 kDa |
| PTM | N-glycosylated and O-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG62791 anti-CD263 / TRAIL R3 antibody [TRAIL-R3-02] FACS image
Flow Cytometry: CD263 transfected HEK-293 cells stained with ARG62791 anti-CD263 / TRAIL R3 antibody [TRAIL-R3-02] at 16 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62791 anti-CD263 / TRAIL R3 antibody [TRAIL-R3-02] FACS image
Flow Cytometry: Separation of CD263 transfected HEK-293 cells (red-filled) from non-transfected HEK-293 cells (black-dashed). Cells were stained with ARG62791 anti-CD263 / TRAIL R3 antibody [TRAIL-R3-02] at 16 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
