ARG53806

anti-CD262 / TRAIL R2 antibody [DR5-01-1] (FITC)

anti-CD262 / TRAIL R2 antibody [DR5-01-1] (FITC) for Flow cytometry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [DR5-01-1] recognizes CD262 / TRAIL R2
Tested Reactivity Hu
Tested Application FACS
Specificity The clone DR5-01-1 recognizes an extracellular domain of TRAIL-R2 (DR5). TRAIL-R2 is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L).
Host Mouse
Clonality Monoclonal
Clone DR5-01-1
Isotype IgG1
Target Name CD262 / TRAIL R2
Antigen Species Human
Immunogen Recombinant fusion protein of human IgG heavy chain and extracellular domain of DR5.
Conjugation FITC
Alternate Names TRICK2A; TRICK2B; KILLER; TRAILR2; TNF-related apoptosis-inducing ligand receptor 2; DR5; CD antigen CD262; TRICK2; CD262; KILLER/DR5; Tumor necrosis factor receptor superfamily member 10B; Death receptor 5; TRAIL-R2; TRAIL receptor 2; TRICKB; ZTNFR9

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8795 Human TNFRSF10B

Swiss-port # O14763 Human Tumor necrosis factor receptor superfamily member 10B

Gene Symbol TNFRSF10B
Gene Full Name tumor necrosis factor receptor superfamily, member 10b
Background TRAIL-R2 (CD262, DR5) is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L).
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including tumor necrosis factor (TNF) and Fas ligand in the TNF family through their death domain containing receptors, TNF receptor 1 (TNFR1) and Fas, respectively. Another member in the TNF family has been identified and designated TRAIL (for TNF related apoptosis inducing ligand) and Apo2L (for Apo2 ligand). Receptors for TRAIL include two death domain containing receptors, DR4 and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. DcR1 (also called TRID), like the related death receptors DR4 and DR5, contains two extracellular cysteine rich domains. However, DcR1 contains no intracellular death domain and is thus incapable of signaling apoptosis. It has been suggested DcR1 is responsible for TRAIL resistance in normal human tissues including heart, placenta, lung, liver, kidney, spleen, and bone marrow. DR5 is a member of the TNF receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor related apoptosis inducing ligand (TNFSF10/TRAIL/APO2L), and transduces apoptosis signal. Studies with FADD deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Calculated MW 48 kDa

Images (1) Click the Picture to Zoom In

  • ARG53806 anti-CD262 / TRAIL R2 antibody [DR5-01-1] (FITC) FACS image

    Flow Cytometry: CD262-transfectants (red) and non-transfectants (black-dashed) stained with ARG53806 anti-CD262 / TRAIL R2 antibody [DR5-01-1] (FITC).

Clone References

Cisplatin and a potent platinum(IV) complex-mediated enhancement of TRAIL-induced cancer cells killing is associated with modulation of upstream events in the extrinsic apoptotic pathway.

ICC/IF / Human

Vondálová Blanárová O et al.
Carcinogenesis.,  (2011)

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