ARG54418

anti-CD253 / TRAIL antibody

anti-CD253 / TRAIL antibody for ICC/IF,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CD253 / TRAIL
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity This antibody recognizes human TRAIL.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD253 / TRAIL
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa 261-277 of human TRAIL (accession no. NP_003801).
Conjugation Un-conjugated
Alternate Names TL2; CD253; Protein TRAIL; TNF-related apoptosis-inducing ligand; TRAIL; CD antigen CD253; Apo-2 ligand; Apo-2L; APO2L; Tumor necrosis factor ligand superfamily member 10

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and Human brain

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8743 Human TNFSF10

Swiss-port # P50591 Human Tumor necrosis factor ligand superfamily member 10

Gene Symbol TNFSF10
Gene Full Name tumor necrosis factor (ligand) superfamily, member 10
Background Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain-containing receptors, TNFR1 and Fas. A novel member of the TNF family has been identified and designated TRAIL (TNF-related apoptosis-inducing ligand) and Apo-2L (Apo-2 ligand). TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain-containing receptors, DR4 and DR5, have been identified as the receptor for TRAIL. Like TNF and Fas ligand, TRAIL induces apoptosis and NF- B activation in many tissues and cell types.
Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 33 kDa
PTM Tyrosine phosphorylated by PKDCC/VLK.

Images (2) Click the Picture to Zoom In

  • ARG54418 anti-CD253 / TRAIL antibody WB image

    Western blot: 10, 2.5, or 1ng of recombinant extracellular domain of TRAIL stained with ARG54418 anti-CD253 / TRAIL antibody at 1 μg/ml dilution.

  • ARG54418 anti-CD253 / TRAIL antibody IHC image

    Immunohistochemistry: Human brain stained with ARG54418 anti-CD253 / TRAIL antibody at 20 μg/ml dilution.