ARG23415
anti-CD25 antibody [PC61.5.3] (PE)
anti-CD25 antibody [PC61.5.3] (PE) for Flow cytometry and Mouse
Immune System antibody; Pre-B Cell Marker antibody
Overview
| Product Description | PE-conjugated Rat Monoclonal antibody [PC61.5.3] recognizes CD25 Rat anti Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | PC61.5.3 |
| Isotype | IgG1 |
| Target Name | CD25 |
| Antigen Species | Mouse |
| Immunogen | B6.1 CTL cell line. |
| Conjugation | PE |
| Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01590 Mouse Interleukin-2 receptor subunit alpha |
|---|---|
| Gene Symbol | IL2RA |
| Gene Full Name | interleukin 2 receptor, alpha |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| Function | Receptor for interleukin-2. [UniProt] |
| Research Area | Immune System antibody; Pre-B Cell Marker antibody |
| Calculated MW | 31 kDa |
Images (1) Click the Picture to Zoom In
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ARG23415 anti-CD25 antibody [PC61.5.3] (PE) FACS image
Flow Cytometry: Figure A. FITC conjugated Rat anti Mouse CD4 and PE-conjugated Rat IgG1 isotype control. Figure B. FITC conjugated Rat anti Mouse CD4 and ARG23415 anti-CD25 antibody [PC61.5.3] (PE). All experiments performed on Murine splenocytes.
